Constructor Detail

• BleachCorrection_ExpoFit

  public BleachCorrection_ExpoFit(ij.ImagePlus imp)

  Parameters:

  imp -

• BleachCorrection_ExpoFit

  public BleachCorrection_ExpoFit(ij.ImagePlus imp,
                                  Roi curROI)

Method Detail

• dcayFitting

  public ij.measure.CurveFitter dcayFitting()

  Fit the mean intensity time series of given ImagePlus in this class. fit equation is 11, parameter from http://rsb.info.nih.gov/ij/developer/api/constant-values.html#ij.measure.CurveFitter.STRAIGHT_LINE

  Returns:

  an instance of CurveFitter

• decayFitting3D

  public ij.measure.CurveFitter decayFitting3D(int zframes,
                                               int tframes)

  Curve fitting for 3D time series is done with average intensity value for wach time point (stack intensity mean is used, so the fitted points = time point, not slice number)

  Parameters:

  zframes -

  tframes -

  Returns:

• calcExponentialOffset

  public double calcExponentialOffset(double a,
                                      double b,
                                      double c,
                                      double x)

  calculate estimated value from fitted "Exponential with Offset" equation

  Parameters:

  a - magnitude (difference between max and min of curve)

  b - exponent, defines degree of decay

  c - offset.

  x - timepoints (or time frame number)

  Returns:

  estimate of intensity at x

• core

  public void core()

  does both decay fitting and bleach correction.